Research Technician (Closed)
*This position is no longer open*
Closing date: 1 Sept 2022
Department: Life Sciences
Location of position: South Kensington Campus
Hours: Full time, fixed term
Salary: £31,773 – £34,680 plus benefits
We are seeking to appoint one experienced Research Technician to join a project that aims to build and improve gene drive systems, based on the use of CRISPR/Cas9 expressed in the germline of the mosquito vectors of malaria, with the ultimate goal of malaria control by decreasing the reproductive potential of mosquito populations. The Crisanti laboratory has already achieved notable successes in recent years, and it is at the forefront in mosquito transgenesis, genome editing and gene drive.
You will help provide technical support to a large team of researchers and other technicians within the Crisanti group at Imperial College that form part of a larger, multi-disciplinary research consortium called Target Malaria. The project is aimed at designing, building and testing novel gene drives through the incorporation of three components: nucleases with improved activity; rational choice of new genetic targets to be affected by the gene drive; modulating and regulating gene drive expression in time and space.
Duties and responsibilities
You will interact on a daily basis with researchers of the group and assist in designing and performing laboratory experiments applied to the generation and molecular analysis of modified mosquitoes, including learning embryo microinjection and genetic crossing experiments.
Your duties will also include assisting in the smooth running and regulatory compliance of the two insectary facilities used by the group, including acting as a caretaker of wild type (on a rota basis to be shared with other technicians in the group) and transgenic mosquito strains, ensuring that mosquito colonies and samples are healthy and available to researchers in the group. Regular support to phenotype assays and to high standards of insectary maintenance are also expected from this post holder.
You must have a bachelor’s degree or equivalent vocational qualification in a biological sciences subject, plus work experience in a relevant technical/scientific roles exhibiting good knowledge of molecular biology techniques and practical methods including RNA extraction, reverse transcription, polymerase chain reaction, gel electrophoresis, DNA purification, blotting and probing, electrophoresis, PCR, qPCR, molecule blotting and probing, CRISPR/Cas9, site-specific mutagenesis, bacteria and cell-lines transfection. Previous experience of working with insects is essential and knowledge of mosquitoes rearing and maintenance is desirable.
Good verbal and written communication skills, accurate record keeping skills and the ability to prioritise tasks to meet deadlines tight deadlines with little supervision are essential. You must be computer literate and a highly motivated, individual with demonstrable ability to learn. You will also be expected to have knowledge of mosquito rearing and maintenance techniques and be able to work in a collaborative manner, be open-minded with a positive approach to develop and apply new concepts, as well as to problem solving. The project may require occasional travel to other collaborating sites within Europe and flexible working hours.
This is a full-time, fixed term position available until 31 May 2024, with the possibility of extension subject to the availability of funding. You will be based at South Kensington Campus.
The College is a proud signatory to the San-Francisco Declaration on Research Assessment (DORA), which means that in hiring and promotion decisions, we evaluate applicants on the quality of their work, not the journal impact factor where it is published. For more information, see https://www.imperial.ac.uk/research-and-innovation/about-imperial-research/research-evaluation/
The College believes that the use of animals in research is vital to improve human and animal health and welfare. Animals may only be used in research programmes where their use is shown to be necessary for developing new treatments and making medical advances. Imperial is committed to ensuring that, in cases where this research is deemed essential, all animals in the College’s care are treated with full respect, and that all staff involved with this work show due consideration at every level. http://www.imperial.ac.uk/research-and-innovation/about-imperial-research/research-integrity/animal-research/